Human PKLR antibody

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.

Product Specifications

Product Name Alternative

Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PKLR (47-574aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1E3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PKLR, ATGA0209-10 µg, ATGA0209-20 µg, ATGA0209-50 µg, ATGA0209-100 µg, ATGA0209-250 µg, ATGA0209-500 µg, ATGA0209-1 mg, ATGA0209-10, ATGA0209-20, ATGA0209-50, ATGA0209-100, ATGA0209-250, ATGA0209-500, ATGA0209-1

References & Citations

Meza NW., et al. (2009) . Mol Ther. 17 (12) :2000-9.; ; Roy MF., et al. (2007) . J Exp Med. 204 (12) :2949-61.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000289

Uniprot Accession Number

P30613

WB Description

The cell lysate of 293T (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Balb-3T3 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Raji cell lysate; ; Lane 4.: K562 cell lysate; ; Lane 5.: A549 cell lysate {ATGA0209-WB2.jpg}

IF Description

ICC/IF analysis of PKLR in Hep3B cells. The cell was stained with ATGA0209 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of PKLR in HeLa cells. The cell was stained with ATGA0209 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0209-IF2.jpg}