Human Adenosine Kinase/ADK antibody

Adenosine kinase (ADK) is the key regulator of adenosine metabolism. Because of the manifold receptor-dependent actions of adenosine, tight regulation of adenosine levels is crucial. The intracellular and extracellular pools of adenosine are in dynamic exchange by equilibrative and concentrative nucleoside transporters, so extracellular concentrations of adenosine are regulated by interplay of theses transporters with intracellular and extracellular enzymes of adenosine metabolism. Thus, the extracellular concentration of adenosine is enhanced by inhibition of equilibrative nucleoside transporters such as S- (4-nitrobenzyl) -6-thioinosine and cannabidiol and, stimulation of extracellular ATP breakdown and inhibition of intracellular adenosine removal.

Product Specifications

Product Name Alternative

ADK, AK, adenosine kinase, adenosine 5'-phosphotransferase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ADK (22-362aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4F8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Adenosine Kinase, ADK, ATGA0206-10 µg, ATGA0206-20 µg, ATGA0206-50 µg, ATGA0206-100 µg, ATGA0206-250 µg, ATGA0206-500 µg, ATGA0206-1 mg, ATGA0206-10, ATGA0206-20, ATGA0206-50, ATGA0206-100, ATGA0206-250, ATGA0206-500, ATGA0206-1

References & Citations

Mathews II , et al. (1998) . Biochemistry 1037 (45) :15607-20.; ; Lindberg B, Klenow H, Hansen K (1967) . J. Biol. Chem. 242 (3) : 350-6.; ; CAPUTTO R (1951) . J. Biol. Chem. 189 (2) : 801-14.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006712

Uniprot Accession Number

P55263

WB Description

The cell lysates of HepG2 and 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of ADK in A549 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of ADK in A549 cells. The cell was stained with ATGA0206 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .