Human pan-RAS antibody

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.

Product Specifications

Product Name Alternative

Neuroblastoma RAS viral (v-ras) oncogene homolog, GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, AV095280, N ras, N ras protein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHuMP00000013879, Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NRAS (1-186aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2G9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

V ras neuroblastoma RAS viral oncogene homolog, Transforming protein N Ras, OTTHuMP00000013879, NS6, NRAS1, N-ras, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, Neuroblastoma RAS viral (v-ras) oncogene homolog, OTTMuSP00000023521, Neuroblastoma RAS viral (v-ras) oncogene homolog, Neuroblastoma RAS viral (v ras) oncogene homolog, N ras protein part 4, N ras, HRAS1, GTPase NRas, AV095280, ALPS4, RAS, ATGA0203-10 µg, ATGA0203-20 µg, ATGA0203-50 µg, ATGA0203-100 µg, ATGA0203-250 µg, ATGA0203-500 µg, ATGA0203-1 mg, ATGA0203-010, ATGA0203-020, ATGA0203-050, ATGA0203-100, ATGA0203-250, ATGA0203-500, ATGA0203-01M

References & Citations

Rocks O, et al. (2006) . Current Opinion in Cell Biology 18 (4) : 351-7.; ; Wennerberg K, et al. (March 2005) . J. Cell. Sci. 118 (Pt 5) : 843-6.; ; Rotblat B, et al. (2008) . Methods Enzymol 439: 467-89.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002515

Uniprot Accession Number

P01111

WB Description

The cell lysates of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (200ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human NRAS protein; ; Lane 2.: Recombinant human KRAS protein; ; Lane 3.: Recombinant human HRAS protein; ; {ATGA0203-WB2.jpg}

FACS Description

Flow cytometry analysis of RAS in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of RAS in HeLa cells. The cell was stained with ATGA0203 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .