Human Aldo-keto Reductase 1C1/AKR1C1 antibody

The human aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) have major roles in pre receptor regulation of progesterone action. They can both convert progesterone to the less potent efficiencies. AKR1C1 and AKR1C3 also act as 3-ketosteroid reductase, and as such they can convert the most potent androgen 5alpha-DHT into 3beta-andorstandiol, which is an estrogen receptor beta ligand, and into the inactive androgen 3alpha-androstnionl, respectively.

Product Specifications

Product Name Alternative

Aldo-keto reductase family 1 member C1, DDH1, DDH, MBAB, DD1, HAKRC, Dihydrodiol dehydrogenase 1, 20-alpha (3-alpha) -hydroxysteroid dehydrogenase, High-affinity hepatic bile acid-binding protein (HBAB)

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AKR1C1 (1-323aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT6D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Aldo-keto Reductase1C1, AKR1C1, ATGA0201-10 µg, ATGA0201-20 µg, ATGA0201-50 µg, ATGA0201-100 µg, ATGA0201-250 µg, ATGA0201-500 µg, ATGA0201-1 mg, ATGA0201-10, ATGA0201-20, ATGA0201-50, ATGA0201-100, ATGA0201-250, ATGA0201-500, ATGA0201-1

References & Citations

L.C. Giudice, et al. (2004) Lancet. 3644: 1789-1799.; ; P.G. Hompes, et al. (2007) Gynecol. Endocrinol. 23: 5-12.; ; K.J. Berkley, et al. (2005) Science 308: 1587-1589.

Other References

Hanada N, et al. Methylation of the KEAP1 gene promoter region in human colorectal cancer. (BMC Cancer. 2012) {https://pubmed.ncbi.nlm.nih.gov/22325485/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_001344

Uniprot Accession Number

Q04828

WB Description

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Recombinant Human AKR1C1; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : Raji cell lysate

FACS Description

Flow cytometry analysis of AKR1C1 in A431 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of AKR1C1 in A431 cells. The cell was stained with ATGA0201 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .