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Human Carbonyl reductase 1/CBR1 antibody

Carbonyl reductase 1 (CBR1) is a NADPH-dependent, monomeric, and cytosolic enzyme belonging to a family of short-chain dehydrogenases/reductases. This protein consists of 277 amino acid residues and is widely distributed in human tissues such as liver, epidermis, stomach, small intestine, kidney, neuronal cells, and smooth muscle fiber. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer drug, doxorubicin. The best substrates of CBR1 are quinones, including ubiquinone-1 and tocophrolquinone (vitamin E) .

Product Specifications

Product Name Alternative

Carbonyl reductase 1, Carbonyl reductase 1, CBR, hCBR1, SDR21C1, CBR1, Carbonyl reductase 1 15 hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl reductase [NADPH] 1.CBR 1, CRN, NADPH dependent carbonyl reductase 1, Prostaglandin 9 ketoreductase, Prostaglandin E (2) 9 reductase.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CBR1 (1-277 aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT4E12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CBR1, Carbonyl reductase 1, Carbonyl reductase 1, CBR, hCBR1, SDR21C1, CBR1, Carbonyl reductase 1 15 hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl reductase [NADPH] 1.CBR 1, CRN, NADPH dependent carbonyl reductase 1, Prostaglandin 9 ketoreductase, Prostaglandin E (2) 9 reductase., ATGA0200-10 µg, ATGA0200-20 µg, ATGA0200-50 µg, ATGA0200-100 µg, ATGA0200-250 µg, ATGA0200-500 µg, ATGA0200-1 mg, ATGA0200-10, ATGA0200-20, ATGA0200-50, ATGA0200-100, ATGA0200-250, ATGA0200-500, ATGA0200-1

References & Citations

Lemieux N, et al. (1993) Genomics. 15 (1) :169-72.; ; Wermuth B, et al. (1986) . Biochem. Pharmacol. 35 (8) : 1277-82

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:1,000.

Scientific Category

Metabolism

NCBI Accession Number

NP_001748

Uniprot Accession Number

P16152

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: 293T cell lysate;

IF Description

ICC/IF analysis of CBR1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-CBR1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .
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