Human CtBP1 antibody

The C-terminal-binding protein (CtBP) was originally identified as a cellular protein that interacts with a C-terminal motif of adenovirus E1A oncoproteins. CtBP is highly conserved in higher eukaryotes. Vertebrates contain two highly homologous CtBP proteins (CtBP1 and CtBP2) while invertebrates contain a single CtBP. CtBP family proteins share significant sequence homology with D-2-hydroxy acid dehydrogenases. Also, CtBP proteins associate with various proteins and functions as a co-repressor of transcription.

Product Specifications

Product Name Alternative

C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CTBP1 (1-440aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT4D6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CtBP1, C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684., ATGA0199-10 µg, ATGA0199-20 µg, ATGA0199-50 µg, ATGA0199-100 µg, ATGA0199-250 µg, ATGA0199-500 µg, ATGA0199-1 mg, ATGA0199-10, ATGA0199-20, ATGA0199-50, ATGA0199-100, ATGA0199-250, ATGA0199-500, ATGA0199-1

References & Citations

Katsanis N, et al. (1998) . Genomics 47 (2) : 294-9.; ; Paliwal S, et al. (March 2006) . Mol. Cell. Biol. 26 (6) : 2360-72.; ; Chinnadurai G (2002) . Mol. Cell 9 (2) : 213-24.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Recommended starting dilution is 1:500

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001319

Uniprot Accession Number

Q13363

WB Description

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: NIH3T3 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: AGS cell lysate ; ; Lane 5.: Raji cell lysate ; ; Lane 6.: A549 cell lysate ; ; Lane 7.: Jurkat cell lysate

IF Description

ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0199-IF1b.jpg} ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0199-IF2a.jpg} {ATGA0199-IF2b.jpg}