Human NELL2 antibody

Protein kinase C-binding protein, NELL2 is expressed abundantly in neural tissues and may play important roles in the development of neural tissues. Also, NELL protein contains six epidermal growth factor (EGF) -like repeat domains that participate in calcium binding.

Product Specifications

Product Name Alternative

Mel91, MEL91 protein, NEL-like protein 2, Nel-related protein 2, NRP2, Protein kinase C-binding protein NELL2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NELL2 (30-258aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT13E7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NELL2, Mel91, MEL91 protein, NEL-like protein 2, Nel-related protein 2, NRP2, Protein kinase C-binding protein NELL2, ATGA0198-10 µg, ATGA0198-20 µg, ATGA0198-50 µg, ATGA0198-100 µg, ATGA0198-250 µg, ATGA0198-500 µg, ATGA0198-1 mg, ATGA0198-10, ATGA0198-20, ATGA0198-50, ATGA0198-100, ATGA0198-250, ATGA0198-500, ATGA0198-1

References & Citations

Kuroda S, et al. (1999) Biochem. Biophys. Res. Commun. 265 (1) : 79-86.; ; Luce MJ, Burrows PD. (1999) Gene 231 (1-2) : 121-6.; ; Watanabe TK, et al. (1997) . Genomics 38 (3) : 273-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Recommended starting dilution is 1:500

Scientific Category

Neuroscience

NCBI Accession Number

NP_006150

Uniprot Accession Number

Q99435

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NELL2 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0198-WB1b.jpg}