Human beta-II Tubulin antibody

Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Product Specifications

Product Name Alternative

TUBB2B, Tubulin beta 2B class Iib, Tubulin beta 2b

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human beta-Tubulin (1-445aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT5B2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

B-Tubulin, DKFZp566F223, FLJ98847, MGC8685, Tubulin beta-2B chain, bA506K6.1, TUBB2B, Tubulin beta 2b, Tubulin beta-2B, ATGA0196-10 µg, ATGA0196-20 µg, ATGA0196-50 µg, ATGA0196-100 µg, ATGA0196-250 µg, ATGA0196-500 µg, ATGA0196-1 mg, ATGA0196-10, ATGA0196-20, ATGA0196-50, ATGA0196-100, ATGA0196-250, ATGA0196-500, ATGA0196-1

References & Citations

Xavier Hubert Jaglin, et al. (2009) Nat Genet, 41 (6) :746-52.; ; Bouwmeester T, et al. (2004) Nat Cell Bio, 6 (2) :97-105.; ; Modig, C, et al. (1999) Cell Motil Cytoskeleton, 42 (4) : 315-330.

Storage Conditions

Can be stored at +4C For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction, Cytoskeleton

NCBI Accession Number

NP_821080

Uniprot Accession Number

Q9BVA1

WB Description

The cell lysates (40ug) and tissue lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: A549 cell lysate; ; Lane 3.: A431 cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: Mouse brain tissue lyate

IF Description

Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red) .Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green) .{ATGA0196-IF2.jpg}Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green) .{ATGA0196-IF3.jpg}