Human NQO2 antibody

Quinone oxidoreductase (NQO1 and NQO2) are cytosolic proteins that catalyze metabolic redaction of quinines and derivates. NQO2 is inhibited by flavones such as quercetin. Also benzo (a) pyrene is another known inhibitor of NQO2. Even though overlapping substrates specificities have been observed for NQO1 and NQO2, such as for CB1954 activation, significant differences exist in relative affinities for the various substrates. The detoxification role of NQO2 has not been found, and it has no known endogenous biological substrates. However, NQO1 plays an important role in the detoxification of various endogenous and exogenous quinones, including estrogen quinines. Also NQO2 has a melatonin-binding site, which may explain the anti-oxidant role of melatonin related with circadian rhythm.

Product Specifications

Product Name Alternative

N-ribosyldihydronicotinamide:quinone reductase 2, NMOR2, NAD (P) H menadione oxidoreductase 2, Dioxin-inducible, NAD (P) H quinone dehydrogenase 2, Quinone reductase 2, QR2, DHQV, DIA6, NRH:quinone oxidoreductase 2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NQO2 (1-231aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1E3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NQO2, ATGA0192-10 µg, ATGA0192-20 µg, ATGA0192-50 µg, ATGA0192-100 µg, ATGA0192-250 µg, ATGA0192-500 µg, ATGA0192-1 mg, ATGA0192-10, ATGA0192-20, ATGA0192-50, ATGA0192-100, ATGA0192-250, ATGA0192-500, ATGA0192-1

References & Citations

Wu K., et al, . (1997) Arch Biochem Biophys. 347 (2) :221-8.; ; Aiswal AK., et al, . (1994) J Biol Chem. 269 (20) :14502-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

AAH06096

Uniprot Accession Number

P16083

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: A549 cell lysate

IF Description

ICC/IF analysis of NQO2 in A549 cells. The cell was stained with ATGA0192 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of NQO2 in HeLa cells. The cell was stained with ATGA0192 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0192-IF2.jpg}