Human ERK1/MAPK3 antibody

Mitogen-activated protein kinase (MAPKs) are a widely conserved family of serine/threonine kinase involved in many cellular programs such as cell proliferation, differentiation, motility, and death. MAPK3 (ERK1) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is on important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK) . Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, PRKM3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MAPK3 protein (1-137aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1A2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, p44 MAPK, p44 ERK1, Mitogen-activated protein kinase 3, Mitogen activated protein kinase 3, Microtubule-associated protein 2 kinase, Microtubule associated protein 2 kinase, MAPK-3, MAPK3, MAPK 3, MAP kinase3, MAP kinase isoform p44, MAP kinase 3, Insulin-stimulated MAP2 kinase, Insulin stimulated MAP2 kinase, HuMKER1A, HS44KDAP, Extracellular signal-regulated kinase 1, Extracellular signal regulated kinase 1, ERT-2, ERT2, ERT 2, ERK-2, ERK2, ERK-1, ERK1, ERK 2, ERK 1, ATGA0191-10 µg, ATGA0191-20 µg, ATGA0191-50 µg, ATGA0191-100 µg, ATGA0191-250 µg, ATGA0191-500 µg, ATGA0191-1 mg, ATGA0191-010, ATGA0191-020, ATGA0191-050, ATGA0191-100, ATGA0191-250, ATGA0191-500, ATGA0191-01M

References & Citations

Meloche S, et al, . Oncogene (2007) 26:3227-39; ; Raman M, et al, . Oncogene (2007) 26:3100-12; ; Roberts PJ, et al, . Oncogene (2007) 26:3291-310; ; Shaul YD, et al, . Seger R. Acta (2007) 1773:1213-26

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_002737.2

Uniprot Accession Number

P27361

WB Description

The Recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: ERK2 recombinant protein ; ; Lane 2.: ERK1 recombinant protein The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate; ; Lane 2.: ERK1 (MAPK3) Transfected 293T lysate{2004010154946741.jpg}

IF Description

ICC/IF analysis of MAPK3 in HeLa cells line, stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:500) with goat anti-mouse IgG-Texas Red conjugate (Red) ICC/IF analysis of MAPK3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0191-IF2.jpg}