Human beta-II Tubulin antibody

The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2b is mainly expressed in developing neurons with a dominant expression during the critical steps of corticogenesis. Also, TUBB2B is a new critical gene involved in neuronal migration. It is present in the nuclei and nucleoplasm, Defects in this gene are a cause of asymmetric polymicrogyria.

Product Specifications

Product Name Alternative

TUBB2B, Tubulin beta 2B class Iib, Tubulin beta 2b

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human beta-Tubulin (1-445aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT5B3

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

B-Tubulin, bA506K6.1, DKFZp566F223, FLJ98847, MGC8685, Tubulin beta-2B chain, tubulin beta 2B, ATGA0187-10 µg, ATGA0187-20 µg, ATGA0187-50 µg, ATGA0187-100 µg, ATGA0187-250 µg, ATGA0187-500 µg, ATGA0187-1 mg, ATGA0187-10, ATGA0187-20, ATGA0187-50, ATGA0187-100, ATGA0187-250, ATGA0187-500, ATGA0187-1

References & Citations

Xavier Hubert Jaglin, et al. (2009) Nat Genet, 41 (6) :746-52.; ; Bouwmeester T, et al. (2004) Nat Cell Bio, 6 (2) :97-105.; ; Modig, C, et al. (1999) Cell Motil Cytoskeleton, 42 (4) : 315-330.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and IHC analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_821080

Uniprot Accession Number

Q9BVA1

WB Description

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain tissue lysate

IHC description

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human beta-Tubulin antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .