Human SUMO 2/3 antibody

SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family of ubiquitin-related proteins. 8-11 kDa that covalently modify various intracellular proteins. It resembles ubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of a variety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residues after the GG region of these precursors by SUMO-specific proteases in maturation is a prerequisite for subsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recent studies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical, biological consequences.

Product Specifications

Product Name Alternative

Ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, SuMO3, SuMO2, SMT3H2, SMT3H1, SMT3B, SMT3A, SMT3 suppressor of mif two 3 homolog 2, SMT3 homolog 2, SMT 3B, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), Small ubiquitin related modifier 2, Small ubiquitin like modifier 2, Sentrin2, MGC117191, HSMT3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SUMO2 (1-93aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT10F1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HSMT 3, HSMT3, MGC117191, Sentrin 2, Sentrin2, Sentrin-2, Small ubiquitin like modifier 2, Small ubiquitin related modifier 2, Small ubiquitin-related modifier 2, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), SMT 3B, SMT3 homolog 2, SMT3 suppressor of mif two 3 homolog 2, SMT3A, SMT3B, SMT3H1, SMT3H2, SuMO 3, SuMO2, SuMO-2, SuMO3, SuMO-3, ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, ATGA0184-10 µg, ATGA0184-20 µg, ATGA0184-50 µg, ATGA0184-100 µg, ATGA0184-250 µg, ATGA0184-500 µg, ATGA0184-1 mg, ATGA0184-010, ATGA0184-020, ATGA0184-050, ATGA0184-100, ATGA0184-250, ATGA0184-500, ATGA0184-01M

References & Citations

Tatsuya Ii, et al. (2007) DNA Repair (Amst), 6 (11) : 1679-1691.; ; Zheng Xu, et al. (2005) Biochem J, 386 (Pt 2) : 325-330.; ; Melchior F. (2000) Annu Rev Cell Dev Biol, 16: 591-626.

Other References

Ham JH, et al. An Immunohistochemical Study on the Expression of SUMO-2/3 in the Colorectal Carcinoma. (Soonchunhyang Med Sci. 2012) {https://jsms.sch.ac.kr/journal/view.php?year=2012&vol=18&no=2&spage=95}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_008868

Uniprot Accession Number

P61956

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: K562 cell lysateThe cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HL-60 cell lysate; ; {ATGA0184-WB2.jpg}The recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human SUMO1 protein; ; Lane 2.: Recombinant human SUMO2 protein; ; Lane 3.: Recombinant human SUMO3 protein; ; {ATGA0184-WB3.jpg}

IHC description

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .

FACS Description

Flow cytometry analysis of SUMO2/3 in jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red) .