Human ERK2/MAPK1 antibody

Mitogen-activated protein kinases (MAPKs) are reported to be critical regulatory factors for the growth andmigration of various cell types including vascular smooth muscle cells (VSMCs) . It has also been reported that the activation of the MAP kinase family, extracellular signal regulated kinases 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) via Src activation is important for AII-induced migration of VSMCs. MAPKs are intracellular signal-transduction pathways that have been shown to play a central role in the development of injury following ischemia in the brain and heart. MAPKs regulate gene expression, which is important in cell injury/repair and proliferation/differentiation.

Product Specifications

Product Name Alternative

PRKM2, PRKM1, p42MAPK, p41MAPK, p41, p40, p38, Mitogen-activated protein kinase 1 MAP kinase 2, Mitogen-activated protein kinase 1, MAPK2, MAPK1, Extracellular Signal Regulated Kinase 2, ERT1, ERK2, ERK,

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MAPK1 (1-360) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1A4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ERK, ERK 2, ERK2, ERT 1, ERT1, Extracellular Signal Regulated Kinase 2, MAPK 1, MAPK 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 1 MAP kinase 2, p38, p40, p41, p41mapk, P42MAPK, PRKM1, PRKM2, ATGA0182-10 µg, ATGA0182-20 µg, ATGA0182-50 µg, ATGA0182-100 µg, ATGA0182-250 µg, ATGA0182-500 µg, ATGA0182-1 mg, ATGA0182-010, ATGA0182-020, ATGA0182-050, ATGA0182-100, ATGA0182-250, ATGA0182-500, ATGA0182-01M

References & Citations

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107 (5) :2037-42.; ; Gesslein B, et al. (2010) Mol Vis,16:392-407.; ; Lai EW, et al. (2004) J Invest Surg, 17:45-53.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 3000. Recommended starting dilution is 1:500

Scientific Category

Cancer

NCBI Accession Number

NP_620407

Uniprot Accession Number

P28482

WB Description

Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green) .