Human Lactate Dehydrogenase A/LDHA antibody

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1) . LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Product Specifications

Product Name Alternative

Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human LDHA (1-332aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1A4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Lactate Dehydrogenase A, LDHA, ATGA0180-10 µg, ATGA0180-20 µg, ATGA0180-50 µg, ATGA0180-100 µg, ATGA0180-250 µg, ATGA0180-500 µg, ATGA0180-1 mg, ATGA0180-10, ATGA0180-20, ATGA0180-50, ATGA0180-100, ATGA0180-250, ATGA0180-500, ATGA0180-1

References & Citations

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107 (5) :2037-42.; ; Xie H, et al. (2009) Mol Cancer Ther, 8 (3) :626-35.; ; Toyoda K, et al. (2010) J Bacteriol, 191 (13) :4251-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_005557

Uniprot Accession Number

P00338

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: A431 cell lysate; ; Lane 4.: HeLa cell lysate;

IF Description

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red) .