Human KCTD15 antibody

KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

Product Specifications

Product Name Alternative

BTB/POZ domain-containing protein KCTD15, MGC25497, MGC2628, potassium channel tetramerisation domain containing 15

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KCTD15 (1-234) purified from E.coli

Clonality

Monoclonal

Isotype

IgG3 κ

Clone

AT4C3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KCTD15, ATGA0178-10 µg, ATGA0178-20 µg, ATGA0178-50 µg, ATGA0178-100 µg, ATGA0178-250 µg, ATGA0178-500 µg, ATGA0178-1 mg, ATGA0178-10, ATGA0178-20, ATGA0178-50, ATGA0178-100, ATGA0178-250, ATGA0178-500, ATGA0178-1

References & Citations

Willer CJ, et al. (2010) Nat. Genet, 41 (1) :24-34.; ; Elks CE, et al. (2010) PLoS Med, 7 (5) :e1000284.; ; Strausberg RL, et al. (2002) Proc. Natl. Acad. Sci. U.S.A, 99 (26) :16899-903.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_076981

Uniprot Accession Number

Q96SI1

WB Description

The cell lysate of HeLa (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : 293T cell lysate; ; Lane 2 : KCTD15 Transfected 293T cell lysate {ATGA0178-WB2.jpg}

IF Description

Immunofluorescence of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red) . Nucleus was stained by Hoechst 33342 (Blue) .