Human MINCLE/CLEC4E antibody

CLEC4E (macrophage inducible C-type lectin, also called MINCLE or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. CLEC4E expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6, 6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB) . Recently it was reported that CLEC4E is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma chain (FcRgamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

Product Specifications

Product Name Alternative

CLEC4E, CLECSF9, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MINCLE (41-219aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT16E3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CLEC 4E, CLEC4E, CLECSF9, C-type lectin domain family 4, member E, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2, ATGA0175-10 µg, ATGA0175-20 µg, ATGA0175-50 µg, ATGA0175-100 µg, ATGA0175-250 µg, ATGA0175-500 µg, ATGA0175-1 mg, ATGA0175-010, ATGA0175-020, ATGA0175-050, ATGA0175-100, ATGA0175-250, ATGA0175-500, ATGA0175-01M

References & Citations

Ishikawa E, et al. (2009) J Exp Med, 206 (13) :2879-88.; ; Matsunaga I, et al. (2009) J Exp Med, 206 (13) :2865-8.; ; Graham LM, et al. (2009) Cytokine, 48 (1-2) :148-55.

Other References

Seifert L, et al. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression. (Nature. 2016) {https://pubmed.ncbi.nlm.nih.gov/27049944/}; ; Zhao X, et al. Activation of C-Type Lectin Receptor and (RIG) -I-Like Receptors Contributes to Proinflammatory Response in Middle East Respiratory Syndrome Coronavirus-Infected Macrophages. (J Infect Dis. 2020) {https://pubmed.ncbi.nlm.nih.gov/31562757/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_055173

Uniprot Accession Number

Q9ULY5

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CLEC4E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : 293T cell lysate; ; Lane 2. : MINCLE transfected 293T cell lysate

IHC description

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human CLEC4E antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .

FACS Description

Flow cytometry analysis of CLEC4E in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

Immunofluorescence of human HeLa cells stained with monoclonal anti-human CLEC4E antibody (1:500) with Texas Red (Red) . Nucleus was stained by Hoechst 33342 (Blue) .