Human KAT2A/GCN5 antibody

GCN5L2, as known as GCN5 and KAT2A is the use of immunoprecipitated GCN5L2 for acetylation to provide the additional components important for acetylation. GCN5L2 can be one of the potential enzymes involved in acetylation in vivo. GCN5L2 is expressed predominantly in the embryo and newborn and is essential for normal embryonic development. Therefore, GCN5L2 appears to be important for differentiation of embryonic-derived preadipocytes. On the one hand, loss of GCN5L2 leads to high incidence of apoptosis in the GCN5L2 mutants that begins before the onset of morphological abnormality.

Product Specifications

Product Name Alternative

STAF97, PCAF-b, Lysine acetyltransferase 2A, Histone succinyltransferase KAT2A, Histone glutaryltransferase KAT2A, Histone acetyltransferase KAT2A, Histone acetyltransferase GCN5, General control of amino-acid synthesis 5-like 2, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GCN5L2 (411-837aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT3G13

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

STAF97, PCAF-b, Lysine acetyltransferase 2A, KAT2A, K (lysine) acetyltransferase 2A, Histone succinyltransferase KAT2A, Histone glutaryltransferase KAT2A, Histone acetyltransferase KAT2A, Histone acetyltransferase GCN5, hGCN5L2, hGCN5, General control of amino-acid synthesis 5-like 2, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, ATGA0174-10 µg, ATGA0174-20 µg, ATGA0174-50 µg, ATGA0174-100 µg, ATGA0174-250 µg, ATGA0174-500 µg, ATGA0174-1 mg, ATGA0174-010, ATGA0174-020, ATGA0174-050, ATGA0174-100, ATGA0174-250, ATGA0174-500, ATGA0174-01M

References & Citations

Wiper-Bergeron N, et al. (2007) Proc Natl Acad Sci U S A, 104 (8) :2703-8.; ; Jacob AL, et al. (2001) J Biol Chem, 276 (40) :37659-64.; ; Xu W, et al. (2000) Nat Genet, 26 (2) :229-232.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_066564

Uniprot Accession Number

Q92830

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KAT2A/GCN5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate