Human IL-32 antibody

The gene encoding IL32 is located on human chromosome 16p13. 3 and is organized into eight exons. IL32 is mainly expressed in natural killer cells, T cells, epithelial cells, and blood monocytes. It can induce the proinflammatory cytokines TNFalpha and IL1beta in murine peritoneal macrophages as well as in phorbol ester-differentiated human THP1 cells. In the inflamed lesions of chronic pancreas, the ductal expression of IL32 was markedly increased. Pancreatic duct cells are the local source of IL32, and IL32 may play an important role in inflammatory responses and pancreatic cancer growth. Recently, a synergism between IL32 and other well characterized players in innate immunity has been documented.

Product Specifications

Product Name Alternative

Interleukin 32, Natural killer cells protein 4, Natural killer cell transcript 4, NK4, Tumor necrosis factor alpha-inducing factor, TAIF, TAIFb, TAIFd

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IL32 (1-131aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2F9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IL32 a, ATGA0170-10 µg, ATGA0170-20 µg, ATGA0170-50 µg, ATGA0170-100 µg, ATGA0170-250 µg, ATGA0170-500 µg, ATGA0170-1 mg, ATGA0170-10, ATGA0170-20, ATGA0170-50, ATGA0170-100, ATGA0170-250, ATGA0170-500, ATGA0170-1

References & Citations

Nishida A, et al. (2009) J Biol Chem, 284 (26) :17868-76.; ; Kobayashi H, et al. (2009) Cytokine, 46 (3) :351-8.; ; Mabilleau G, et al. (2009) PLoS One, 4 (1) :e4173.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_001012651

Uniprot Accession Number

P24001

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-32 alpha antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: IL-32 alpha Transfected 293T cell lysate;

IF Description

ICC/IF analysis of IL32 in Jurkat cells. The cell was stained with ATGA0170 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .