Human Thymidylate synthase/TYMS antibody

The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dMTP) is an essential step for the formation of thymine nucleotides. This process is catalyzed by thymidylate synthase (TS or TYMS) . TYMS is an intracellular enzyme that provides the sole de novo source of thymidylate in activity highest proliferating cells. Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) . 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head and neck. TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers.

Product Specifications

Product Name Alternative

HST422, TMS, TS, d TMP synthase, EC 2.1.1.45, MGC88736, Thymidylate synthase, Thymidylate synthetase, Tsase, TYMS, TYMS protein, Tyms thymidylate synthetase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant TYMS (1-313aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT21S5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TYMS, ATGA0169-10 µg, ATGA0169-20 µg, ATGA0169-50 µg, ATGA0169-100 µg, ATGA0169-250 µg, ATGA0169-500 µg, ATGA0169-1 mg, ATGA0169-10, ATGA0169-20, ATGA0169-50, ATGA0169-100, ATGA0169-250, ATGA0169-500, ATGA0169-1

References & Citations

Shahrokni A, et al., (2009) Clin Colorectal Cancer 8 (4) :231-4.; ; Henriquez-Hernandez LA, et al., (2009) Oncol Rep 22 (6) :1425-33.; ; Colavito D, et al., (2009) Cancer Chemother Pharmacol 64 (6) :1195-200.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001062

Uniprot Accession Number

P04818

WB Description

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TYMS (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant human TYMS protein ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: Jurkat cell lysate ; ; Lane 4.: MCF7 cell lysate

FACS Description

Flow cytometry analysis of TYMS in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

HeLa cells were stained with monoclonal anti-TYMS antibody (Green) . Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue) . ICC/IF analysis of TYMS in HeLa cells. The cell was stained with ATGA0169 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0169-IF2.jpg}