Human E2-EPF/UBE2S antibody

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.

Product Specifications

Product Name Alternative

Ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, Ubiquitin-conjugating enzyme S, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S, Ubiquitin conjugating enzyme E2 24 kD, Ubiquitin conjugating enzyme E2S

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human UBE2S (1-222aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2C12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

UBE2S, ATGA0165-10 µg, ATGA0165-20 µg, ATGA0165-50 µg, ATGA0165-100 µg, ATGA0165-250 µg, ATGA0165-500 µg, ATGA0165-1 mg, ATGA0165-10, ATGA0165-20, ATGA0165-50, ATGA0165-100, ATGA0165-250, ATGA0165-500, ATGA0165-1

References & Citations

Ye Y, et al. (2009) Nat Rev Mol Cell Biol, 10 (11) :755-64.; ; Windheim M, et al. (2008) Biochem J, 409 (3) :723-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_055316.2

Uniprot Accession Number

Q16763

WB Description

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: UBE2S Transfected 293T cell lysate;

FACS Description

Flow cytometry analysis of UBE2S in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green) .