Human FUS2/NAA80 antibody

Vertebrate FUS2 genes, which are known to be putative tumor suppressor gene, contain several important domains such as the catalytic N-acetyltransferase (NAT) domain. NAT domain is essential enzymes involved in several sophisticated cellular processes such as N-acetylation, O-acetylatin. NAT enzymes may be involved in susceptibility to cancer including colorectal cancer because of the presence of carcinogenic heterocyclic amines in some cooked foods. FUS2 was physically localized to the cytoplasm. Also, FUS2 showed the actin dependent movement, closely related to the polarization in the budding yeast, Saccharomyces cerevisiae.

Product Specifications

Product Name Alternative

Protein fus 2, Protein fusion-2, FuS-2, NAT6, N acetyltransferase 6

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant FUS2 (1-308aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2F4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Protein fusion-2, FuS2, FuS-2, NAT6, FuS 2, N acetyltransferase 6, Protein fus 2, Protein fusion 2, Protein fus-2, ATGA0164-10 µg, ATGA0164-20 µg, ATGA0164-50 µg, ATGA0164-100 µg, ATGA0164-250 µg, ATGA0164-500 µg, ATGA0164-1 mg, ATGA0164-010, ATGA0164-020, ATGA0164-050, ATGA0164-100, ATGA0164-250, ATGA0164-500, ATGA0164-01M

References & Citations

Duh FM, et al., (2004) Mol Cell Probes 18 (1) :39-44.; ; Gatphayak K, et al., (2004) Gene 337:105-111.; ; Zegerman P, et al., (2000) Oncogene 19 (1) :161-163.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_036323

Uniprot Accession Number

Q93015

WB Description

The cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUS2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: FUS2 Transfected 293T cell lysate;

FACS Description

Flow cytometry analysis of FUS2 in Balb/3T3 cells. The cell was stained with ATGA0164 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .