Human CRABP1 antibody

CRABP1 (cellular retinoic acid-binding protein 1) is a member of specific carrier proteins for members of the vitamin A family. CRABP1 is assumed to play an important role in retinoic acid-mediated differentiation and proliferation processes. CRABP1 is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid. It is constitutively expressed and is believed to have different functions in the cell than the related CRABP2. Interestingly, Sytenol A bakuchiol has very specific receptor specificity over retinol and has no effect on the RAR-beta and RAR-gamma receptors and down-regulates CRABP1.

Product Specifications

Product Name Alternative

Cellular retinoic acid binding protein 1, CRABP, CRABP-I, CRABPI, RBP5, Cellular retinoic acid binding protein 1 CRABP 1, CRABP I, RBP 5, Retinoic acid binding protein I cellular

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CRABP1 (1-137aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1A1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CRABP1, ATGA0162-10 µg, ATGA0162-20 µg, ATGA0162-50 µg, ATGA0162-100 µg, ATGA0162-250 µg, ATGA0162-500 µg, ATGA0162-1 mg, ATGA0162-10, ATGA0162-20, ATGA0162-50, ATGA0162-100, ATGA0162-250, ATGA0162-500, ATGA0162-1

References & Citations

Wang L, et al. (1997) J Biol Chem, 272 (3) :1541-7.; ; . Nezzar H, et al. (2007) Mol Vis, 13:1641-50.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004369

Uniprot Accession Number

P29762

WB Description

The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Eye Tissue lysateThe cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; {2004010134347560.jpg}The cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: CRABP1 Transfected 293T cell lysate; ; {2004010134867354.jpg}

FACS Description

Flow cytometry analysis of CRABP1 in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of CRABP1 in Balb/3T3 cells. The cell was stained with ATGA0162 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .