Human BRCC36/BRCC3 antibody

BRCC36 (BRCA1/BRCA2-containing complex, subunit 36) is a holoenzyme complex containing BRCA1, BRCA2. BRCC36 is an ubiquitin E3 ligase complex that has a role in DNA repair and enhances cellular survival following DNA damage. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint.

Product Specifications

Product Name Alternative

BRCA1/BRCA2-containing complex subunit 3, chromosome X open reading frame 53, C6.1A

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant BRCC36 (1-316aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3B1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BRCC36, ATGA0159-10 µg, ATGA0159-20 µg, ATGA0159-50 µg, ATGA0159-100 µg, ATGA0159-250 µg, ATGA0159-500 µg, ATGA0159-1 mg, ATGA0159-10, ATGA0159-20, ATGA0159-50, ATGA0159-100, ATGA0159-250, ATGA0159-500, ATGA0159-1

References & Citations

Wang Bin, et al., (2007) Proc Natl Acad Sci USA 104 (52) :20759-20763.; ; Chen Xiaowei, et al., (2006) Cancer Research 66:5039-5046.; ; Dong Y, et al., (2003) Mol Cell 12 (5) :1087-1099.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_077308

Uniprot Accession Number

P46736

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: NIH3T3 cell lysate;

IF Description

ICC/IF analysis of BRCC36 in HeLa cells. The cell was stained with ATGA0159 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .