Human G6PD antibody

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.

Product Specifications

Product Name Alternative

Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6PDH

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human G6PD (35-506aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2F6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

G6PD, ATGA0158-10 µg, ATGA0158-20 µg, ATGA0158-50 µg, ATGA0158-100 µg, ATGA0158-250 µg, ATGA0158-500 µg, ATGA0158-1 mg, ATGA0158-10, ATGA0158-20, ATGA0158-50, ATGA0158-100, ATGA0158-250, ATGA0158-500, ATGA0158-1, Glucose-6-phosphate 1-dehydrogenase, zwf, Glucose-6-phosphate 1-dehydrogenase G6PD, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p

References & Citations

Huang Y., et al, (2008) Mol Genet Metab. 93 (1) :44-53.; ; Zimny A., et al. (2003) Pol Arch Med Wewn. 110 (5) :1327-33.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_001035810.1

Uniprot Accession Number

P11413

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human G6PD antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Jurkat cell lysate;

IF Description

ICC/IF analysis of G6PD in HeLa cells. The cell was stained with ATGA0158 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .