Human TDP1 antibody

Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that catalyzes the hydrolysis of a phophodiester bond between a tyrosine residue and a DNA 3' phosphate. This protein is a member of the phospholipase D (PLD) superfamily and contains two PLD phosphodiesterase domains. Mutations in TDP1 are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1) by interfering with DNA transcription or by inducting apoptosis in postmitotic neurons.

Product Specifications

Product Name Alternative

Tyrosyl-DNA phosphodiesterase 1, FLJ11090, SCAN1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TDP1 (1-298aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1F2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TDP1, ATGA0157-10 µg, ATGA0157-20 µg, ATGA0157-50 µg, ATGA0157-100 µg, ATGA0157-250 µg, ATGA0157-500 µg, ATGA0157-1 mg, ATGA0157-10, ATGA0157-20, ATGA0157-50, ATGA0157-100, ATGA0157-250, ATGA0157-500, ATGA0157-1

References & Citations

Dexheimer TS, et al., (2008) Anticancer Agents Med Chem. 8 (4) :381-9.; ; EI-Khamisy SF, Caldecott KW., (2006) Mutagenesis. 21 (4) :219-24.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_060789

Uniprot Accession Number

Q9NUW8

WB Description

The cell lysate of MCF7 (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;

FACS Description

Flow cytometry analysis of TDP1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of TDP1 in Hep3B cells. The cell was stained with ATGA0157 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .