Human HAX-1 antibody

HAX1 is known to associate with hematopoietic cell-specific Lyn substrate 1 (HS1), one of the substrates of receptor-coupled tyrosine kinases activated during clonal expansion and deletion in lymphoid cells. It also interacts with the product of the polycystic kidney disease 2 (PKD2) gene and with the F-actin-binding protein, cortactin. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.

Product Specifications

Product Name Alternative

HCLS1 associated protein X-1, HS1BP1, HCLSBP1, HCLS1 (and PKD2) associated protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human HAX1 (1-279aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT3C5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HAX1, ATGA0156-10 µg, ATGA0156-20 µg, ATGA0156-50 µg, ATGA0156-100 µg, ATGA0156-250 µg, ATGA0156-500 µg, ATGA0156-1 mg, ATGA0156-10, ATGA0156-20, ATGA0156-50, ATGA0156-100, ATGA0156-250, ATGA0156-500, ATGA0156-1

References & Citations

Klein C, et al., (2007) Nat Genet. 39 (1) :86-92.; ; Yin H, et al., (2001) Cytokine. 15 (3) :122-37.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_006109

Uniprot Accession Number

O00165

WB Description

The cell lysate of HeLa (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HAX-1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HAX-1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Raji cell lysate; ; {ATGA0156-WB2.jpg}

IF Description

ICC/IF analysis of HAX-1 in HeLa cells. The cell was stained with ATGA0156 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .