Human TBCA antibody

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.

Product Specifications

Product Name Alternative

Tubulin folding cofactor A, Tubulin folding cofactor A, chaperonin cofactor a, tubulin specific chaperone a, TBCA, Tubulin folding cofactor A CFA, Co chaperonin associated with a & b tubulin, Cofactor A, TCP1 chaperonin cofactor A, Tubulin cofactor a

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant TBCA (1-108aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1A5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TBCA, ATGA0155-10 µg, ATGA0155-20 µg, ATGA0155-50 µg, ATGA0155-100 µg, ATGA0155-250 µg, ATGA0155-500 µg, ATGA0155-1 mg, ATGA0155-10, ATGA0155-20, ATGA0155-50, ATGA0155-100, ATGA0155-250, ATGA0155-500, ATGA0155-1

References & Citations

Nolasco S, et al., (2005) FEBS Lett 579 (17) :3515-3524.; ; Tian G, et al., (1996) Cell 86 (2) :287-296.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004598

Uniprot Accession Number

O75347

WB Description

The cell lysate of LNCap (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate; ; {ATGA0155-WB2.jpg}

FACS Description

Flow cytometry analysis of TBCA in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IF Description

ICC/IF analysis of TBCA in A549 cells. The cell was stained with ATGA0155 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of TBCA in HeLa cells. The cell was stained with ATGA0155 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010129463677.jpg}