Human RNH1 antibody

Ribonuclease inhibitor 1 (RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.

Product Specifications

Product Name Alternative

Ribonuclease/angiogenin inhibitor 1, ribonuclease/angiogenin inhibitor, RAI

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant Ribonuclease inhibitor 1 (RNH1) (7-461aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1H23

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

RNH1, ATGA0154-10 µg, ATGA0154-20 µg, ATGA0154-50 µg, ATGA0154-100 µg, ATGA0154-250 µg, ATGA0154-500 µg, ATGA0154-1 mg, ATGA0154-10, ATGA0154-20, ATGA0154-50, ATGA0154-100, ATGA0154-250, ATGA0154-500, ATGA0154-1

References & Citations

Park J.H., et al., (2008) Cytotechnology 57 (1) :93-99.; ; Johnson R.J., et al., (2007) J Mol Biol 368 (2) :434-449.; ; Fu P, et al., (2005) Cancer Gene Ther 12 (3) :268-275.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_002930

Uniprot Accession Number

P13489

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RNH1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: HepG2 cell lysate;

IF Description

ICC/IF analysis of RNH1 in PC3 cells. The cell was stained with ATGA0154 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .