Human LITAF antibody

Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) mediates the expression of inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced processes. LITAF binds to STAT6B, a member of the STAT6 family forming a complex on the TNF-alpha promoter that modulates TNF activity. High levels of expression of LITAF mRNA have been observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen.

Product Specifications

Product Name Alternative

Lipopolysaccharide-induced TNF factor, PIG7, SIMPLE, FLJ38636, TP53I7

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human LITAF (1-161aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1F9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

LITAF, ATGA0152-10 µg, ATGA0152-20 µg, ATGA0152-50 µg, ATGA0152-100 µg, ATGA0152-250 µg, ATGA0152-500 µg, ATGA0152-1 mg, ATGA0152-10, ATGA0152-20, ATGA0152-50, ATGA0152-100, ATGA0152-250, ATGA0152-500, ATGA0152-1

References & Citations

Atucchi A, et al., (2006) Inflamm Bowel Dis 13 (1) :120-121.; ; Tang X, et al., (2005) Proc Natl Acad Sci USA 102 (14) :5132-5137.; ; Tang X, et al., (2003) Proc Natl Acad Sci USA 100 (7) :4096-4101.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004853

Uniprot Accession Number

Q99732

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LITAF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate;

IF Description

ICC/IF analysis of LITAF in LnCap cells. The cell was stained with ATGA0152 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .