Aequorea victoria GFP antibody

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Product Specifications

Product Name Alternative

Green fluorescent protein

Host

Mouse

Antigen Species

GFP

Reactivity

Aequorea victoria

Immunogen

Recombinant GFP (1-238aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2G5

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GFP, ATGA0148-10 µg, ATGA0148-20 µg, ATGA0148-50 µg, ATGA0148-100 µg, ATGA0148-250 µg, ATGA0148-500 µg, ATGA0148-1 mg, ATGA0148-10, ATGA0148-20, ATGA0148-50, ATGA0148-100, ATGA0148-250, ATGA0148-500, ATGA0148-1

References & Citations

Phillips G (2001) FEMS Microbiol Lett 204 (1) :9-18.; ; Tsien R (1998) Annu Rev Biochem 67:509-544.; ; Prasher, et al., (1992) Gene 111 (2) :229-233.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Fusion Tag & Detection

Uniprot Accession Number

P42212

WB Description

The recombinant proteins were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human GFP protein (20ng) ; ; Lane 2.: Recombinant human GFP protein (40ng) ; ; Lane 3.: Recombinant human GFP protein (80ng) The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GFP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: GFP transfected 293T cell lysate {ATGA0148-WB2.jpg}