Human GAS7 antibody

Growth arrest-specific 7, also known as GAS7, is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development by promoting maturation and morphological differentiation of cerebellar neurons. Inhibition of GAS7 production in terminally differentiating cultures of embryonic murine cerebellum impedes neurite outgrowth. Conversely, the hyper-expression of GAS7 may play an important role in the initiation and development of human osteosarcoma.

Product Specifications

Product Name Alternative

Growth arrest-specific 7 isoform b, KIAA0394, Growth arrest-specific 7, isoform b Growth arrest specific 7, GAS 7, GAS7, MGC1348, MLL/GAS7 fusion protein.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GAS7 (1-416aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4H8

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GAS7, Growth arrest-specific 7 isoform b, KIAA0394, Growth arrest-specific 7, isoform b Growth arrest specific 7, GAS 7, GAS7, MGC1348, MLL/GAS7 fusion protein., ATGA0147-10 µg, ATGA0147-20 µg, ATGA0147-50 µg, ATGA0147-100 µg, ATGA0147-250 µg, ATGA0147-500 µg, ATGA0147-1 mg, ATGA0147-10, ATGA0147-20, ATGA0147-50, ATGA0147-100, ATGA0147-250, ATGA0147-500, ATGA0147-1

References & Citations

Chao CC, et al., (2005) J Neurosci Res. 81 (2) :153-62; ; She BR, et al., (2002) Exp Cell Res. 273 (1) :34-44

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.

Scientific Category

Neuroscience

NCBI Accession Number

NP_958836

Uniprot Accession Number

O60861

WB Description

The extracts of mouse brain (50 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAS7 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of GAS7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of GAS7 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0147-IF2.jpg}