Human HOMER1 antibody

Homer1 (Homer protein homolog 1) belongs to Homer family of proteins that regulate signal transduction through, and the trafficking of, glutamate receptors, as well as maintain and regulate extracellular glutamate levels in corticolimbic brain regions. The Homer1 is enriched at excitatory synapses and binds group 1 metabotropic glutamate receptors (mGluRs) .

Product Specifications

Product Name Alternative

HOMER, Homer protein homolog 1, HOMER1, HOMER1A, HOMER1B, HOMER1C, Neuronal immediate early gene, SYN47, Ves 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Homer1 (1-354aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1F3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HOMER, HOMER 1, HOMER 1A, HOMER 1B, HOMER 1C, Homer protein homolog 1, Homer protein homolog1, HOMER1, HOMER1A, HOMER1B, HOMER1C, Neuronal immediate early gene, Neuronal immediate early gene 1, SYN 47, SYN47, Ves 1, Ves1, ATGA0146-10 µg, ATGA0146-20 µg, ATGA0146-50 µg, ATGA0146-100 µg, ATGA0146-250 µg, ATGA0146-500 µg, ATGA0146-1 mg, ATGA0146-010, ATGA0146-020, ATGA0146-050, ATGA0146-100, ATGA0146-250, ATGA0146-500, ATGA0146-01M

References & Citations

Szumlinski KK, et al., (2008) Biochem Pharmacol. 75 (1) :112-33.; ; Roche KW, et al., (1999) J Biol Chem.274 (36) :25953-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004263

Uniprot Accession Number

Q86YM7

WB Description

The tissue lysate (50 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HOMER1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain Tissue lysate

IF Description

ICC/IF analysis of HOMER1 in U87MG cells. The cell was stained with ATGA0146 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .