Human ORF73/HHV8 antibody

ORF73, HHV8, ATGA0145-10 µg, ATGA0145-20 µg, ATGA0145-50 µg, ATGA0145-100 µg, ATGA0145-250 µg, ATGA0145-500 µg, ATGA0145-1 mg, ATGA0145-10, ATGA0145-20, ATGA0145-50, ATGA0145-100, ATGA0145-250, ATGA0145-500, ATGA0145-1

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ORF73/HHV8 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate (40ug) ; ; Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate (40ug) ; ; Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug) {ATGA0145-WB2.jpg}

Paraffin embedded sections of Kaposi's sarcoma tissue were incubated with anti-ORF73/HHV8 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)