Human ORF73/HHV8 antibody

ORF73 protein of human herpesvirus 8 (HHV-8) is a kaposi's sarcoma-associated herpesvirus (kSHV) latent protein which has been suspected of playing a crucial role in modulating viral and cellular gene expression. ORF73 is associated with cellular chromatin and stays on the chromosomes during cell division. It maintains the viral genomes during cell division by tethering the viral episomes to the chromosomes. It binds directly to replication origin recognition complexes (ORCs) that are primarily associated with the terminal repeat (TR) region of the HHV-8 genome.

Product Specifications

Product Name Alternative

Human herpesvirus 8 ORF73, Kaposi's sarcoma-associated herpesvirus

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ORF73/HHV8 (122-329aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT4C11

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ORF73, HHV8, ATGA0145-10 µg, ATGA0145-20 µg, ATGA0145-50 µg, ATGA0145-100 µg, ATGA0145-250 µg, ATGA0145-500 µg, ATGA0145-1 mg, ATGA0145-10, ATGA0145-20, ATGA0145-50, ATGA0145-100, ATGA0145-250, ATGA0145-500, ATGA0145-1

References & Citations

Kedes D.H., et al. (1997) Journal of Clinical Investigation. 100: 2610-2606.; ; Verma SC., et al (2006) Journal of Virology 80: 3445-3458.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

AAB62657

Uniprot Accession Number

O40947

WB Description

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ORF73/HHV8 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate (40ug) ; ; Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate (40ug) ; ; Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug) {ATGA0145-WB2.jpg}

IHC description

Paraffin embedded sections of Kaposi's sarcoma tissue were incubated with anti-ORF73/HHV8 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)