Human FKBP52/FKBP4 antibody

FkBP4, also known as FkBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes.

Product Specifications

Product Name Alternative

FK506 binding protein 4, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, HBI, Hsp 56, HSP binding immunophilin, p52, p59 protein, Peptidylprolyl cis trans isomerase, PPIase, Rotamase, T cell FK506 binding protein 59kD

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FkBP4 (1-459aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT4D3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

FK506 binding protein 4, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, FKBP4, FKBP52, FKBP52 protein, FKBP59, HBI, Hsp 56, HSP binding immunophilin, Hsp56, p52, p59 protein, Peptidyl prolyl cis trans isomerase, Peptidylprolyl cis trans isomerase, PPIase, Rotamase, T cell FK506 binding protein 59kD, ATGA0142-10 µg, ATGA0142-20 µg, ATGA0142-50 µg, ATGA0142-100 µg, ATGA0142-250 µg, ATGA0142-500 µg, ATGA0142-1 mg, ATGA0142-010, ATGA0142-020, ATGA0142-050, ATGA0142-100, ATGA0142-250, ATGA0142-500, ATGA0142-01M

References & Citations

Ostrow KL, et al., (2009) Clin Cancer Res. 15 (4) :1184-91.; ; Ma D, et al., (2008) J Biol Chem. 283 (38) :25963-70.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Redox Proteins

NCBI Accession Number

NP_002005

Uniprot Accession Number

Q02790

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP52 /FKBP4 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: MCF7 cell lysate;

IHC description

Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP52 /FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of FKBP52 /FKBP4 in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IF Description

ICC/IF analysis of FKBP52 /FKBP4 in HeLa cells. The cell was stained with ATGA0142 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .