Human Cyclophilin F/PPIF antibody

Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-transisomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

Product Specifications

Product Name Alternative

Peptidylprolyl isomerase F, Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Mitochondrial cyclophilin, CyP-M, Rotamase F

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPIF (30-207aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1F5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cyclophilin D, Cyclophilin F, Cyp D, CYP3, CypD, Cyp-D, hCyP3, mitochondral, Peptidyl prolyl cis trans isomerase, Peptidylprolyl isomerase F, Peptidylprolyl isomerase F (cyclophilin F), Peptidylprolyl isomerase F Cyclophilin 3, PPIase, PPIF, Rotamase, ATGA0139-10 µg, ATGA0139-20 µg, ATGA0139-50 µg, ATGA0139-100 µg, ATGA0139-250 µg, ATGA0139-500 µg, ATGA0139-1 mg, ATGA0139-010, ATGA0139-020, ATGA0139-050, ATGA0139-100, ATGA0139-250, ATGA0139-500, ATGA0139-01M

References & Citations

Bowles KR, et al., (2000) Genomics 67 (2) :109-127; ; Bergsma DJ, et al., (1991) J Biol Chem 266 (34) :23204-23214

Other References

Kon N, et al. A small-molecule DS44170716 inhibits Ca 2+-induced mitochondrial permeability transition. (Sci Rep. 2017) {https://pubmed.ncbi.nlm.nih.gov/28634393/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_005720

Uniprot Accession Number

P30405

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: PC3 cell lysate

IHC description

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .{ATGA0139-IHC2.jpg}

IF Description

ICC/IF analysis of PPIF in HeLa cells. The cell was stained with ATGA0139 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .