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Human Retinol Binding Protein 4/RBP4 antibody

Retinol binding protein 4 (RBP4) belongs to the lipocalin family and is the specific carrier for retinol in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.

Product Specifications

Product Name Alternative

Plasma retinol-binding protein, PRBP, RBP

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human RBP4 (19-201aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2B4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

RBP4, Retinol-Binding Protein4, ATGA0136-10 µg, ATGA0136-20 µg, ATGA0136-50 µg, ATGA0136-100 µg, ATGA0136-250 µg, ATGA0136-500 µg, ATGA0136-1 mg, ATGA0136-10, ATGA0136-20, ATGA0136-50, ATGA0136-100, ATGA0136-250, ATGA0136-500, ATGA0136-1

References & Citations

Tan Bk, et al., (2007) J Clin Endocrinol Metab 92 (7) :2590-2597.; ; Yao-Borengasser A, et al., (2007) J Clin Endocrinol Metab 92 (7) :2430-2432.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_006735

Uniprot Accession Number

P02753

WB Description

The HepG2 cell lysate (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RBP4 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RBP4 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: RBP4 Transfected 293T cell lysate; ; {ATGA0136-WB2.jpg}

FACS Description

Flow cytometry analysis of RBP4 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of RBP4 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .
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