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Human CRABP2 antibody

The cellular retinoic acid-binding protein II (CRABP-II) is involved in the conversion of vitamin A into its intracellular active form retinoic acid, which regulate the genes responsible for lipid metabolism and adipocyte differentiation. CRABP2 gene is located on chromosome 1q21-23 and this region has been linked with related disorders such as familial combined hyperlipidemia (FCHL) and type 2 diabetes mellitus.

Product Specifications

Product Name Alternative

Cellular retinoic acid binding protein 2, RBP6, CRABP-II, Cellular retinoic acid binding protein 2, CRABP2, RBP6, CRABP-II, Cellular retinoic acid binding protein 2 Cellular retinoic acid binding protein II, CRABP II, CRABPII

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CRABP2 (1-138aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2E11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CRABP2, ATGA0134-10 µg, ATGA0134-20 µg, ATGA0134-50 µg, ATGA0134-100 µg, ATGA0134-250 µg, ATGA0134-500 µg, ATGA0134-1 mg, ATGA0134-10, ATGA0134-20, ATGA0134-50, ATGA0134-100, ATGA0134-250, ATGA0134-500, ATGA0134-1

References & Citations

Astrom, A., et al (1991) . J. Biol. Chem. 266 (26), 17662-17666.; ; Gupta, A., et al (2006) . Cancer Res. 66 (16), 8100-8108.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001869

Uniprot Accession Number

P29373

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP2 antibody (1:250) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : MCF-7 cell lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : 293T cell lysate; ; Lane 2. : CRABP2 Transfected 293T cell lysate{2004010114908304.jpg}

IF Description

ICC/IF analysis of CRABP2 in HeLa cells. The cell was stained with ATGA0134 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of CRABP2 in MCF7 cells. The cell was stained with ATGA0134 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0134-IF2.jpg}
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