Human NFATC1 antibody

Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.

Product Specifications

Product Name Alternative

Nuclear factor of activated T-cells, cytosolic component 1 isoform A, NF-ATC, NFATc, NFAT2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NFATc1 (428-716aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1C3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NFATC1, ATGA0133-10 µg, ATGA0133-20 µg, ATGA0133-50 µg, ATGA0133-100 µg, ATGA0133-250 µg, ATGA0133-500 µg, ATGA0133-1 mg, ATGA0133-10, ATGA0133-20, ATGA0133-50, ATGA0133-100, ATGA0133-250, ATGA0133-500, ATGA0133-1

References & Citations

Akimzhanov A, et al., (2008) Am J Pathol 172 (1) :215-224.; ; Asagiri M, et al., (2005) J Exp Med 202 (9) :1261-1269.; ; Rao A, et al., (1997) Annu Rev Immunol 15:707-747.

Other References

Vizza D, et al. Exposure to nerve growth factor worsens nephrotoxic effect induced by Cyclosporine A in HK-2 cells. (PLoS One. 2013) {https://pubmed.ncbi.nlm.nih.gov/24244623/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_765978

Uniprot Accession Number

O95644

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NFATC1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 3.: NFATC1 Transfected 293T cell lysate;

FACS Description

Flow cytometry analysis of NFATC1 in Jurkat cells. The cell was stained with ATGA0133 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of NFATC1 in HeLa cells. The cell was stained with ATGA0133 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .