Human 14-3-3 theta antibody

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.

Product Specifications

Product Name Alternative

YWHAQ, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta, Protein theta

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human 14-3-3 tau (1-245aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1A1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

1433 theta, ATGA0132-10 µg, ATGA0132-20 µg, ATGA0132-50 µg, ATGA0132-100 µg, ATGA0132-250 µg, ATGA0132-500 µg, ATGA0132-1 mg, ATGA0132-10, ATGA0132-20, ATGA0132-50, ATGA0132-100, ATGA0132-250, ATGA0132-500, ATGA0132-1

References & Citations

Liu YC, et al., (1996) J Biol Chem 271:14591-14595.; ; Xiao B, et al., (1995) Nature 376:188-191.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006817

Uniprot Accession Number

P27348

WB Description

The cell lysate of Jurkat (20 µg) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Recombinant Human YWHAZ; ; Lane 2. : Recombinant Human YWHAB ; ; Lane 3. : Recombinant Human YWHAE ; ; Lane 4. : Recombinant Human YWHAH; ; Lane 5. : Recombinant Human YWHAG; ; Lane 6. : Recombinant Human SFN ; ; Lane 7. : Recombinant Human YWHAQ; ; {ATGA0132-WB2.jpg}The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : MDA-MB-21 cell lysate ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : A431 cell lysate ; ; Lane 4. : NIH3T3 cell lysate ; ; Lane 5. : 293T cell lysate ; ; Lane 6. : HepG2 cell lysate ; ; {ATGA0132-WB3.jpg}

IF Description

ICC/IF analysis of 14-3-3 tau in HeLa cells. The cell was stained with ATGA0132 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .