Human FABP7/B-FABP antibody

FABP7 (Fatty acid binding protein 7) is also known as brain fatty acid-binding protein (B-FABP) and is a member of fatty acid-binding proteins (FABPs) which are a family of small, highly conserved, cytoplasmic proteins to bind long-chain fatty acids and other hydrophobic ligands. FABP7 is expressed in radial glia by the activation of Notch receptors and binds DHA with the highest affinity among all of FABPs.

Product Specifications

Product Name Alternative

Fatty acid binding protein 7, Fatty acid binding protein 7, FABP7, Fatty acid binding protein 7 B FABP, BFABP, BLBP, Brain lipid binding protein, DKFZp547J2313, FABP 7, FABPB, Fatty acid binding protein 7 brain, Fatty acid binding protein brain, OTTHuMP00000017119, Mammary derived growth inhibitor related, MRG

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FABP7 (1-132aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1D1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

FABP7, B-FABP, ATGA0131-10 µg, ATGA0131-20 µg, ATGA0131-50 µg, ATGA0131-100 µg, ATGA0131-250 µg, ATGA0131-500 µg, ATGA0131-1 mg, ATGA0131-10, ATGA0131-20, ATGA0131-50, ATGA0131-100, ATGA0131-250, ATGA0131-500, ATGA0131-1

References & Citations

Borchers T, et al., (1997) Prostaglandins Leukot. Essent. Fatty Acids. 57 (1) :77-84.; ; Liu Rz, et al., (2003) Eur J Biochem. 270 (4) :715-25.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001437

Uniprot Accession Number

O15540

WB Description

The Mouse tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP7 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Testis tissue lysate The tissue lysate of mouse brain (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP7 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0131-WB2.jpg}

IF Description

ICC/IF analysis of FABP7 in U87MG cells. The cell was stained with ATGA0131 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with ATGA0131 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0131-IF2.jpg}