Human KLF4 antibody

KLF4, which is also known as the gut-enriched kruppel-like factor (GkLF), is a zinc finger-containing transcription factor that belongs to the kruppel-like family of transcription factors. kLF4 plays important roles during the proliferation and differentiation of epithelial cells. Especially, it is found predominantly in gut and has been shown to be expressed during growth arrest. kLF4 is also involved in cell cycle control since expression of kLF4 inhibits DNA synthesis by blocking the G1/S phase of the cell cycle.

Product Specifications

Product Name Alternative

Krueppel-like factor 4, EZF, GKLF

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KLF4 (1-170aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4E6

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KLF4, ATGA0129-10 µg, ATGA0129-20 µg, ATGA0129-50 µg, ATGA0129-100 µg, ATGA0129-250 µg, ATGA0129-500 µg, ATGA0129-1 mg, ATGA0129-10, ATGA0129-20, ATGA0129-50, ATGA0129-100, ATGA0129-250, ATGA0129-500, ATGA0129-1

References & Citations

Yan FF, et al., (2008) Med Hypotheses. 70 (4) :845-7.; ; Cho YG, et al., (2007) APMIS. 115 (7) :802-8.

Other References

Petrova A, et al. Induced Pluripotent Stem Cell Differentiation and Three-Dimensional Tissue Formation Attenuate Clonal Epigenetic Differences in Trichohyalin. (Stem Cells Dev. 2016) {https://pubmed.ncbi.nlm.nih.gov/27460132/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_004226.3

Uniprot Accession Number

O43474

WB Description

The recombinant protein (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human KLF4 protein

FACS Description

Flow cytometry analysis of KLF4 in A549 cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .Flow cytometry analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .{ATGA0129-FC2.jpg}