Human Carbonyl reductase 3/CBR3 antibody

Carbonyl reductase 3 (CBR3) is one of several monomeric NADPH-dependent oxidoreductases. This protein catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. It also contains three exons spanning 11. 2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. Some studies suggest that it mediates 9-cis-retinoic acid-induced cytostatis and is a potential prognostic marker for oral malignancy.

Product Specifications

Product Name Alternative

Carbonyl reductase 3, CBR3, NADPH-dependent carbonyl reductase, Carbonyl reductase (NADPH) 3, EC 1.1.1.184, hCBR3, Carbonyl reductase (NADPH) 3 EC 1.1.1.184, NADPH dependent carbonyl reductase 3, SDR21C2, Short chain dehydrogenase/reductase family 21C member 2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CBR3 (1-277aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT7E8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CBR3, ATGA0126-10 µg, ATGA0126-20 µg, ATGA0126-50 µg, ATGA0126-100 µg, ATGA0126-250 µg, ATGA0126-500 µg, ATGA0126-1 mg, ATGA0126-10, ATGA0126-20, ATGA0126-50, ATGA0126-100, ATGA0126-250, ATGA0126-500, ATGA0126-1

References & Citations

Ohkura-Hada S., et al. (2008) . Open Dent J. 2: 78-88. ; ; Miura T., et al. (2009) . Life Sci. 85 (7-8) : 303-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_001227

Uniprot Accession Number

O75828

WB Description

The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: SW480 cell lysate; ; Lane 7.: Mouse brain tissue lysate

IF Description

ICC/IF analysis of CBR3 in HeLa cells. The cell was stained with ATGA0126 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .