Human OSTF1 antibody

Osteoclast-stimulating factor (OSTF1) is an intracellular signaling protein, highly expressed by osteoclasts (OCL) . Indirectly, it enhances osteoclast (OCL) formation and bone resorption through a cellular signal transduction cascade by possibly interacting with c-Src or other c-Src-related proteins. OSTF1 contains a short proline-rich N-terminal region, an SH3 domain, three ankyrin repeats and an aspartate-rich C-terminal region, suggesting that it is potentially involved in protein-protein interactions.

Product Specifications

Product Name Alternative

Osteoclast stimulating factor 1, SH3P2, OSF, Osteoclast stimulating factor 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human OSTF1 (1-217aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT9G4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

OSTF1, ATGA0125-10 µg, ATGA0125-20 µg, ATGA0125-50 µg, ATGA0125-100 µg, ATGA0125-250 µg, ATGA0125-500 µg, ATGA0125-1 mg, ATGA0125-10, ATGA0125-20, ATGA0125-50, ATGA0125-100, ATGA0125-250, ATGA0125-500, ATGA0125-1

References & Citations

Kurihara N., et al. (2001) J. Biol. Chem. 276 (44) : 41035-9. ; ; Reddy S., et al. (1999) J Cell Physiol. 177 (4) : 636-45.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

AAH07459

Uniprot Accession Number

Q92882

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: MCF7 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: LnCap cell lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: OSTF1 Transfected 293T cell lysate {ATGA0125-WB2.jpg}

FACS Description

Flow cytometry analysis of OSTF1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of OSTF1 in MCF7 cells. The cell was stained with ATGA0125 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of OSTF1 in A431 cells. The cell was stained with ATGA0125 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0125-IF2.jpg}