Human 14-3-3 epsilon antibody

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Product Specifications

Product Name Alternative

YWHAE, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon, 14-3-3E, HEL2, KCIP-1, MDCR, MDS

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human14-3-3 epsilon (1-255aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT4F8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

1433 epsilon, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein epsilon polypeptide, YWHAE, MDS, MDCR, KCIP-1, FLJ45465, 14-3-3 epsilon, Tyrosine 3/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, epsilon polypeptide 14 3 3 E, 14 3 3E, KCIP 1, KCIP1, Mitochondrial import stimulation factor L subunit, Protein kinase C inhibitor protein1, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide., ATGA0124-10 µg, ATGA0124-20 µg, ATGA0124-50 µg, ATGA0124-100 µg, ATGA0124-250 µg, ATGA0124-500 µg, ATGA0124-1 mg, ATGA0124-10, ATGA0124-20, ATGA0124-50, ATGA0124-100, ATGA0124-250, ATGA0124-500, ATGA0124-1

References & Citations

Oriente F., et al. (2005) J Biol Chem. 280 (49) : 40642-9.; ; Conklin D., et al. (1995) PNAS. 92 (17) : 7892-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_006752

Uniprot Accession Number

P62258

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: Mouse Brain Tissue lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 14-3-3 Zeta Recombinant Protein ; ; Lane 2.: 14-3-3 Beta Recombinant Protein ; ; Lane 3.: 14-3-3 Epsilon Recombinant Protein ; ; Lane 4.: 14-3-3 Eta Recombinant Protein ; ; Lane 5.: 14-3-3 Gamma Recombinant Protein ; ; Lane 6.: 14-3-3 Sigma Recombinant Protein ; ; Lane 7.: 14-3-3 Tau Recombinant Protein {ATGA0124-WB2.jpg}

IF Description

ICC/IF analysis of 14-3-3 epsilon in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .{ATGA0124-IF1b.jpg} ICC/IF analysis of 14-3-3 epsilon in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0124-IF2a.jpg}{ATGA0124-IF2b.jpg}