Human IL-6 antibody

Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length that plays critical roles in host defense, immune response and hematopoiesis. IL-6 produced by T cells, macrophages, fibroblasts, endothelial and other cells. IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-Beta, is important for developing Th17 responses. The IL-6 receptor is a trimeric complex composed of an IL-6-specific a chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.

Product Specifications

Product Name Alternative

Interleukin-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IFNB2, HGF, HSF

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant IL-6 (30-212aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1H6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

VLAB, VLA-4 subunit bet, VLA4 subunit bet, VLA 4 subunit bet, MSK12, MSK 12, MDF2, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta1, Integrin beta 1, IL-6, IL6, IL 6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, GP IIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0123-10 µg, ATGA0123-20 µg, ATGA0123-50 µg, ATGA0123-100 µg, ATGA0123-250 µg, ATGA0123-500 µg, ATGA0123-1 mg, ATGA0123-010, ATGA0123-020, ATGA0123-050, ATGA0123-100, ATGA0123-250, ATGA0123-500, ATGA0123-01M

References & Citations

Heinrich P.C., et al. (1998) Biochem J. 334: 297-314.; ; Heinrich P.C., et al. (1998) Z Ernahrungswiss. 37: 43-49.; ; Jones S.A. (2005) J Immunol. 175: 3463-3468.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_000591

Uniprot Accession Number

P05231

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-6 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: Raw264.7 cell lysate ; ; Lane 4.: PC3 cell lysate ; ; Lane 5.: THP-1 cell lysate ; ; Lane 6.: A549 cell lysate ; ; Lane 7.: MCF7 cell lysate ; ; Lane 8.: K562 cell lysate

FACS Description

Flow cytometry analysis of IL-6 in LNCaP cells. The cell was stained with ATGA0123 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .Flow cytometry analysis of IL-6 in HeLa cells. The cell was stained with ATGA0123 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .{2004010104258138.jpg}

IF Description

ICC/IF analysis of IL-6 in A549 cells. The cell was stained with ATGA0123 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of IL-6 in HeLa cells. The cell was stained with ATGA0123 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010104255381.jpg}ICC/IF analysis of IL-6 in Hep3B cells. The cell was stained with ATGA0123 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010104258221.jpg} {ATGA0123-IF3b.jpg}