Human KCTD15 antibody

KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

Product Specifications

Product Name Alternative

BTB/POZ domain-containing protein KCTD15, MGC25497, MGC2628

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KCTD15 (1-159aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2B11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KCTD15, ATGA0121-10 µg, ATGA0121-20 µg, ATGA0121-50 µg, ATGA0121-100 µg, ATGA0121-250 µg, ATGA0121-500 µg, ATGA0121-1 mg, ATGA0121-10, ATGA0121-20, ATGA0121-50, ATGA0121-100, ATGA0121-250, ATGA0121-500, ATGA0121-1

References & Citations

Elks CE, et al. (2010) PLoS Med. 7 (5) : e1000284.; ; Strausberg RL, et al. (2002) Proc. Natl. Acad. Sci. U.S.A, 99 (26) : 16899-903.; ; Willer CJ, et al. (2010) Nat. Genet, 41 (1) : 24-34.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_076981

Uniprot Accession Number

Q96SI1

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : HeLa cell lysate; ; Lane 2 : NIH/3T3 cell lysate; ; Lane 3 : K562 cell lysate

FACS Description

Flow cytometry analysis of KCTD15 in HeLa cells. The cell was stained with ATGA0121 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .