Human TDP-43/TARDBP antibody

TAR DNA binding protein (TARDBP), also known as TDP-43, is a cellular protein. TARDBP was identified as the disease accumulating protein in patients with frontotemporal lobar degeneration (FTLD) with ubiquitin inclusions (FTLD-U) and in amyotrophic lateral sclerosis (ALS) . TARDBP functions as a DNA-binding protein and specifically binds to the TAR DNA sequence motifs of HIV. Via this association with TAR motifs, TARDBP acts as a transcriptional repressor and inhibits HIV-1 transcription. In addition, this protein regulates alternate splicing of the cystic fibrosis transmembrane receptor (CFTR) gene.

Product Specifications

Product Name Alternative

TAR DNA binding protein, TDP-43, ALS10, TAR DNA-binding protein 43, TDP43

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TARDBP (1-260aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

K1B8

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TDP43, TARDBP, ATA0834-10 µg, ATA0834-20 µg, ATA0834-50 µg, ATA0834-100 µg, ATA0834-250 µg, ATA0834-500 µg, ATA0834-1 mg, ATA0834-10, ATA0834-20, ATA0834-50, ATA0834-100, ATA0834-250, ATA0834-500, ATA0834-1

References & Citations

Nicola JR, et al., (2008) PLoS Genet 4 (9) :e1000193.; ; Kabashi E, et al., (2008) Nat Genet 40 (5) :572-574.; ; Ou SH, et al., (1995) J Virol 69:3584-3596. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_031401

Uniprot Accession Number

Q13148

WB Description

The cell lysates (20 µg) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TARDBP (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate;

IHC description

Paraffin embedded sections of human prostate cancer tissue were incubated with anti-human TARDBP (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Paraffin embedded sections of human seminoma tissue were incubated with anti-human TARDBP (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {ATA0834-IHC1b.jpg}

FACS Description

Flow cytometry analysis of TARDBP in HeLa cells. The cell was stained with ATA0834 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .