Human GRP94/HSP90B1 antibody

Tumor rejection antigen 1 (TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) . TRA1 is a marker protein of these stem cells.

Product Specifications

Product Name Alternative

94 kDa glucose-regulated protein, 94 kDa glucose-regulated protein, HSP90B1, GP96, TRA1, Tumor rejection antigen 1, GRP94, 94 kDa glucose-regulated protein 94 kDa glucose regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 9, Heat shock protein 90 kDa beta member 1, Stress inducible tumor rejection antigen GP96, Tumor rejection antigen gp96.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TRA1 (676-803aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

2H3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Gp96, HSP90B1, TRA1, ATA0623-10 µg, ATA0623-20 µg, ATA0623-50 µg, ATA0623-100 µg, ATA0623-250 µg, ATA0623-500 µg, ATA0623-1 mg, ATA0623-10, ATA0623-20, ATA0623-50, ATA0623-100, ATA0623-250, ATA0623-500, ATA0623-1

References & Citations

Wulf GM, et al., (2002) J Biol. Chem. 277 (50) :47976-47979.; ; Hamdane M, et al., (2002) J Mol Neurosci. 19 (3) :275-287.; ; Zheng H, et al., (2002) Nature 419 (6909) :853-857.; ; Lu, k.P. et al., (1996) Nature 380 (6574) :544-547.; ; Campbell, H.D, et al., (1997) Genomics 44 (2) :157-162.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_003290

Uniprot Accession Number

P14625

WB Description

The cell lysates of hES (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA1 (1:500) . Protein were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : ES cell lysate

FACS Description

Flow cytometry analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .