Streptavidin antibody

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Product Specifications

Product Name Alternative

Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2.

Host

Mouse

Antigen Species

Human

Immunogen

Recombinant Streptavidin (25-183aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

1C2

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Streptavidin, AST0904-10 µg, AST0904-20 µg, AST0904-50 µg, AST0904-100 µg, AST0904-250 µg, AST0904-500 µg, AST0904-1 mg, AST0904-10, AST0904-20, AST0904-50, AST0904-100, AST0904-250, AST0904-500, AST0904-1

References & Citations

Mark Howarth, et al. (2006) Nature Methods. 3:267-73.; ; Holmberg A., et al. (2005) Electrophoresis. 26 (3) :501-10.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Protein Marker

NCBI Accession Number

CAA27265

Uniprot Accession Number

P22629

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Streptavidin antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Streptavidin protein